The retained stones and flukes in the biliary system are still vexing to most surgeons because of its considerable morbidity and mortality. For the purpose of removing the retained stones and flukes in the biliary system nonsurgically, many authors have performed T-tube irrigations with physiochemical solutions such as ethyl alcohol, ethyl ether ethyl alcohol(2: 1), ethyl ether, heparin, 0.01% benzalkonium chloride solution and other solutions.
This study was undertaken to investigate the effects of physiochemical agents CO. 9% NaCl solution, ethyl alcohol (25% & 50%), ethyl ether-ethyl alcohol, ethyl ether solution) on liver tissues of healthy rabbits. The capacity of the bile duct system of normal rabbits(weight: 2.8±0.5kg) was estimated by infusion of Biligrafin(75%) through catheters in common bile duct
A polyethylene catheter of gauge No. 20 was inserted into the common bile duct of each rabbit, then 2 cc of 0. 9% physiologic saline solution, ethyl alcohol solution(25% & 50%), ethyl ether-ethyl alcohol solution, ethyl ether solution were infused through the catheter in each group.
From six days after the operation, physiochemical agents were infused through catheter in common bile duct once a day (total number of infusion in each group: 2, 5, 10, 15, 30 times). The rabbits were sacrificed for the study of the histopathological findings of the liver, and the following results were obtained:
1. In the control group which was administered ten times of 2 cc of 0.9% sodium chloride solution, the histopathological changes appeared for the first time in the form of mild focal necrosis in hepatic cells and mild fibrosis in bile duct system. Moderate to severe necrosis and fibrosis in hepatic cells and bile duct system appeared in the group of thirty times of administrations.
2. In the 25% ethyl alcohol administered group which was given five times of 2 cc., the histopathological changes appeared for the first time in the form of mild focal necrosis in hepatic cells and mild fibrosis in bile duct system. The degree of histopathological changes of the hepatic cells and bile duct system generally was proportional to the number of drug administrations. The centrilobular necrosis was similar to carbon tetrachloride intoxication appeared in the subgroup of the ten times of administrations.
3. In the 50% ethyl alcohol administered group which was given two times of 2cc., the histopathological changes appeared for the first time in the form of mild focal necrosis in hepatic cells and mild fibrosis in bile duct system. The tissue damage generally was proportional to the times of drug administration.
4. In the 2:1 ethyl ether-ethyl alcohol administered group which was given two times of 2cc., the histopathological changes appeared for the first time in the form of mild focal necrosis in hepatic cells and bile duct system. In this group diffuse hepatic cell necrosis, generally proportional to times of drug administrations, was more prominent than focal hepatic cell necrosis.
5. In the pure ethyl ether administered group, rabbits died from once or twice administration of ethyl ether 2 cc with convulsive seizure tachypnea, cardiac arrest. Ethyl ether was very toxic when it was infused through common bile duct in rabbits. Diffuse hemorrhagic necrosis in hepatic cells was shown with once or twice administration.
6. It is to be inferred that diluted ethyl alcohol, e.g. 25% ethyl alcohol solution, could several times be used for the purpose of removing the flukes in the bilary system.
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